MALAT1 Mediates α-Synuclein Expression through miR-23b-3p to Induce Autophagic Impairment and the Inflammatory Response in Microglia to Promote Apoptosis in Dopaminergic Neuronal Cells

Figure 2

MALAT1 acts as a miR-23b-3p sponge to regulate α-synuclein expression (MN9D cells). (a) StarBase predicts the targeting binding site between MALAT1 and miR-23b-3p. (b) Dual luciferase reporter assay was used to verify the targeting binding relationship (MALAT1 and miR-23b-3p), vs. NC-mimic. (c) The expression of miR-23b-3p was detected by RT-qPCR, vs. si-NC and ^# vs. pcDNA. (d) StarBase predicts the targeting binding site between miR-23b-3p and α-synuclein. (e) Dual luciferase reporter assay was used to verify the targeting binding relationship (miR-23b-3p and α-synuclein), vs. NC-mimic. (f) The expression of α-synuclein was detected by western blot, vs. NC-mimic and ^# vs. NC-inhibitor. (g) The expression of α-synuclein was detected by western blot, vs. NC-pcDNA, ^# vs. pcDNA-MALAT1+NC-mimic, and ^▲ vs. pcDNA-MALAT1+sh-NC. (h) The level of α-synuclein was detected by ELISA, vs. NC-pcDNA, ^#### vs. pcDNA-MALAT1+NC-mimic, and ^▲▲▲▲ vs. pcDNA-MALAT1+sh-NC.

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