MALAT1 Mediates α-Synuclein Expression through miR-23b-3p to Induce Autophagic Impairment and the Inflammatory Response in Microglia to Promote Apoptosis in Dopaminergic Neuronal Cells

Figure 4

Effect of α-synuclein on autophagy and the inflammatory response of microglia. (a) The expression of α-synuclein in BV-2 cells was detected by immunofluorescence. Scale  px. (b) The level of MIP-1α was detected by ELISA, vs. control and ^#### vs. supernatant 1. (c) The level of TNF-α was detected by ELISA, vs. control and ^#### vs. supernatant 1. (d) The level of IL-6 was detected by ELISA, vs. control and ^#### vs. supernatant 1. (e) The level of TNF-α was detected by ELISA, vs. control and ^#### vs. supernatant 1. (f) The level of INF-γ was detected by ELISA, vs. control and ^#### vs. supernatant 1. (g) NF-κB p65 nuclear translocation assay, scale  px. (h) The expressions of LC3 II/I, Beclin 1, and p62 were detected by western blot, , , and vs. control and ^#, ^##, ^###, and ^#### vs. supernatant 1 (note: supernatant 1 group: the supernatant of MN9D cells overexpressing MALAT1 was cocultured with BV2 cells; supernatant 2 group: the supernatant of MN9D cells cotransfected with pcDNA-MALAT1+miR-23b-3p mimic was cocultured with BV2; supernatant 3 groups: the supernatant of MN9D cells cotransfected with pcDNA-MALAT1+sh-α-synuclein was cocultured with BV2).

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