Protease-Activated Receptor-2 and Phospholipid Metabolism Analysis in Hyperuricemia-Induced Renal Injury

Figure 4

The effects on PI3K/AKT/NF-κB pathway in HK-2 cells induced by uric acid (720 μM). HK-2 cells were treated with pCDNA-PAR2 and siPAR2. (a) RT–PCR examined the expression of PAR2, PI3K, AKT, and NF-κB genes. (b) Western blotting determined the expression of PAR2, PI3K, AKT, and NF-κB proteins. (c) Quantitative values of the relative expression levels of PAR2, PI3K, AKT, and NF-κB. (d) The levels of pro-IL-1β, IL-1β, IL-6, MCP-1, TNF-α, and TGF-β in the cell culture supernatant were determined by ELISA assay. Data are presented as means ± standard deviation. n = 3 per group; ns, not significant. .

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