Dimethyl Fumarate Protects against Lipopolysaccharide- (LPS-) Induced Sepsis through Inhibition of NF-κB Pathway in Mice

Figure 1

DMF effects on LPS-induced NO synthesis and iNOS expression. (a) Chemical structure of DMF. (b, c) Inhibition of LPS-stimulated NO synthesis and iNOS expression by DMF. LPS or LPS as well as various concentrations of DMF (10, 20, 40, and 80 µM) were used to treat RAW264.7 cells. On x-aixs, the first 0 corresponds to the blank group and the second 0 corresponds to the LPS stimulation group (without DMF). NO synthesis was evaluated by Griess reagent and expression of iNOS was performed by western blot. The protein levels of iNOS were statistical analyzed by semi-quantitation method. The values represent the mean ± SD of three separate experiments and Student’s t-test was utilized for evaluating the differences in the mean values. Suggests significant difference between the indicated two groups ().

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