Dimethyl Fumarate Protects against Lipopolysaccharide- (LPS-) Induced Sepsis through Inhibition of NF-κB Pathway in Mice

Figure 5

Effects of DMF on the LPS-induced NF-κB signaling pathway. RAW264.7 cells were pretreated with varying concentrations of DMF before stimulation with LPS. (a) The cell signaling proteins, NF-κB p65, IκB, IKK, and their phosphorylated forms, were detected by western blot analysis with appropriate antibodies. (b) The protein levels of pIKK, IKK, pIκB, IκB, p- p65, and p65 were statistical analyzed by semi-quantitation method. On x-aixs, the first 0 corresponds to the blank group and the second 0 corresponds to the LPS stimulation group (without DMF). The values represent the mean ± SD of three separate experiments and Student’s t-test was utilized for evaluating the differences in the mean values. Suggests significant difference between the indicated two groups ().

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