Key Roles of p53 Signaling Pathway-Related Factors GADD45B and SERPINE1 in the Occurrence and Development of Gastric Cancer

Figure 8

GADD45B overexpression or SERPINE1 silencing inhibits the biological characteristics of GC cells. (a) RT-qPCR was used to detect the expression of GADD45B or SERPINE1 in AGS cells. (b) Colony formation assay was used to detect the number of AGS cell clones after GADD45B overexpression or SERPINE1 silencing. (c) The migration rate of AGS cells in response to GADD45B overexpression or SERPINE1 silencing detected by scratch test (scale bar: 100 μm). (d) The number of invading AGS cells in response to GADD45B overexpression or SERPINE1 silencing detected by transwell assay (scale bar: 50 μm). (e) The apoptotic rate of AGS cells in response to GADD45B overexpression or SERPINE1 silencing detected by flow cytometry. (f) The protein expression of PCNA, Bcl-2, and Bax in AGS cells in response to GADD45B overexpression or SERPINE1 silencing detected by western blot assay. vs. oe-NC or sh-NC group. The measurement data were expressed as mean ± standard deviation. An unpaired t-test was used for comparison between the two groups. The cell experiment was repeated three times.

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