ANXA1sp Protects against Sepsis-Induced Myocardial Injury by Inhibiting Ferroptosis-Induced Cardiomyocyte Death via SIRT3-Mediated p53 Deacetylation

Figure 3

Erastin eliminates the protective effect of ANXA1sp against LPS-induced H9C2 cell death, inflammation, and oxidative stress. H9C2 cells were assigned to control, LPS, LPS+ANXA1sp, and LPS+ANXA1sp+erastin groups. (a) CCK-8 for H9C2 cell viability in each group. (b) LDH level in H9C2 cell supernatant from each group. (c–e) ELISA for TNF-α, IL-1β, and IL-6 levels in H9C2 cell supernatant. (f–i) ROS, MDA, SOD, and GSH levels in H9C2 cell supernatant. The experiments were repeated three times (). Data were presented as . All statistical analyses were performed using Student’s -test or one-way ANOVA. ; ; .

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