ANXA1sp Protects against Sepsis-Induced Myocardial Injury by Inhibiting Ferroptosis-Induced Cardiomyocyte Death via SIRT3-Mediated p53 Deacetylation

Figure 4

ANXA1sp promotes p53 deacetylation by enhancing SIRT3 expression. (a, b) WB for SIRT3, ac-p53, and p53 protein levels in H9C2 cells from control, LPS, or LPS+ANXA1sp groups. (c) Co-IP for interaction between SIRT3 and p53 in H9C2 cells from control or LPS groups. (d) Western blotting for H9C2 cells transfected with si-NC or si-SIRT3. (e) Western blotting for ac-p53 protein level in H9C2 cells from control, LPS, LPS+ANXA1sp, or LPS+ANXA1sp+si-SIRT3 groups. The experiments were repeated three times (). Data were presented as . All statistical analyses were performed using Student’s t-test or one-way ANOVA. ; ; .

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