Inhibition of miR-146a Expression and Regulation of Endotoxin Tolerance by Rhesus Theta-Defensin-1

Figure 1

RTD-1 inhibits expression of miR-146a in LPS-stimulated cells. THP-1 cells were treated with 100 ng/ml LPS and RTD-1 at the indicated concentrations for 20 h. The medium was analyzed by TNF-α ELISA (a), and the RNA was analyzed by qRT-PCR for expression of miR-146a (b), and RNU6-2 (c) after normalizing to SNORD68 expression. Data shown are the results of 3 experiments; compared to LPS treatment, two-tailed, paired test. Fold change was calculated with respect to vehicle-treated control in (b) and (c).

(a)

(b)

(c)