Inhibition of miR-146a Expression and Regulation of Endotoxin Tolerance by Rhesus Theta-Defensin-1

Figure 2

RTD-1 does not affect miR-146a stability. THP-1 cells were incubated with 10 ng/ml LPS for 18 h to induce the expression of miR-146a. The cells were then washed and treated with vehicle or 10 μg/ml RTD-1 for 4 h. The RNA was then analyzed by qRT-PCR for miR-146a and SnoRD68 expression after normalizing it to RNU6-2 expression. The average of the 2 experiments is shown. Fold change was calculated with respect to control.