Inhibition of miR-146a Expression and Regulation of Endotoxin Tolerance by Rhesus Theta-Defensin-1

Figure 4

RTD-1 inhibits the establishment of endotoxin tolerance. THP-1 cells were incubated with 10 ng/ml LPS and RTD-1 for 20 h as indicated. Cells were washed and restimulated with 100 ng/ml LPS for 4 h. TNF-α secretion in the medium was analyzed by ELISA after primary stimulation (white columns) and secondary stimulation (black columns). The average of 3 experiments is shown; NS: nonsignificant, two-tailed, paired test. Primary LPS exposure suppresses endotoxin tolerance.