Inhibition of miR-146a Expression and Regulation of Endotoxin Tolerance by Rhesus Theta-Defensin-1

Figure 5

RTD-1 inhibits endotoxin tolerance by regulating NF-κB activity. THP-1 Dual cells were treated with 10 ng/ml LPS, LPS+10 μg/ml RTD-1, or PBS for 18 h. The cells were washed and restimulated with vehicle control or 100 ng/ml LPS for ~22 h. Supernatant SEAP activity was measured by the QUANTI-Blue assay and the fold change between LPS and vehicle-treated secondary stimulation from 3 experiments was plotted. one-tailed, paired test.