Inhibition of miR-146a Expression and Regulation of Endotoxin Tolerance by Rhesus Theta-Defensin-1

Figure 6

Effect of RTD-1 on miR-146a expression and endotoxin tolerance. (a) LPS stimulation of cells leads to activation of the NF-κB pathway and miR-146a expression. IRAK1 is targeted by miR-146a which inhibits further stimulation of the TLR pathway. (b) RTD-1 inhibits activation of the NF-κB pathway which leads to inhibition of miR-146a expression. Suppression of miR-146a expression stabilizes IRAK1 and enables a cellular proinflammatory response as observed by TNF-α secretion during secondary stimulation with LPS.