Influence of Inflammatory Cytokines IL-1β and IFNγ on Sarcoplasmic Aggregation of p62 and TDP-43 in Myotubes

Figure 2

TDP-43 localization with IL-1β + IFNγ treatment. (a) Images of TDP-43 distribution within untreated control myotubes, examples of cells with a nuclear only (blue arrowhead) or nuclear and sarcoplasmic (yellow arrowhead) TDP-43 distribution. Example of sarcoplasmic only (pink arrowhead) TDP-43 distribution. scale bar = 100 µm. (b) immunofluorescent images of TDP-43 localization in control and IL-1β + IFNγ treated myotubes. scale bar = 100 µm. (c) There was no difference between the control and IL-1β + IFNγ cytokine groups for TDP-43 distribution via two-way ANOVA. n = 5 myogenic donors.

(a)

(b)

(c)