Influence of Inflammatory Cytokines IL-1β and IFNγ on Sarcoplasmic Aggregation of p62 and TDP-43 in Myotubes

Figure 3

Sarcoplasmic TDP-43 aggregates and p62 puncta with IL-1β + IFNγ. (a) Representative images of TDP-43 and p62 within control and IL-1β + IFNγ-treated myotubes. There was no difference between control or IL-1β + IFNγ for TDP-43 aggregate frequency relative to cell area (b) or aggregate size (c). Relative p62 puncta frequency was not different between control and IL-1β + IFNγ treatment (d), however, with IL-1β + IFNγ p62 puncta were significantly larger () (e). There was no difference in colocalization of TDP-43 aggregates with p62 puncta between control and IL-1β + IFNγ (f). Student’s T-test or Mann–Whitney U test. n = 6 myogenic donors.

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