Influence of Inflammatory Cytokines IL-1β and IFNγ on Sarcoplasmic Aggregation of p62 and TDP-43 in Myotubes

Figure 5

Sarcoplasmic TDP-43 aggregation and p62 puncta with IL-1β or IFNγ. (a) Representative images of TDP-43 and p62 distribution in control, IL-1β, or IFNγ-treated myotubes. There was no difference between control and IL-1β or control and IFNγ for TDP-43 aggregate frequency relative to cell area (b) or aggregate size (b). p62 relative particle frequency (d) or size (e) was not different between control and IL-1β or control and IFNγ. The percentage of TDP-43 aggregates that colocalized with p62 puncta was not different between control and IL-1β or control and IFNγ (f). One-way ANOVA or Krustal–Walis test. n = 5 myogenic donors.

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