GATA6-AS1 via Sponging miR-543 to Regulate PTEN/AKT Signaling Axis Suppresses Cell Proliferation and Migration in Gastric Cancer

Figure 6

GATA6-AS1 suppresses the progression of GC by regulating PTEN/AKT signaling axis. (a) QRT-PCR was implemented to detect PTEN level in GC cells transfected with pcDNA3.1/GATA6-AS1 and sh-PTEN#1/#2/#3. (b) Western blot was applied to evaluate the protein level of PTEN in GC cells after the transfection with pcDNA3.1/GATA6-AS1 and sh-PTEN#1/#2/#3 (left panels). Quantification of western blot results was shown in the bar graphs (right panels). (c) Western blot was applied to detect the protein level of AKT and phosphorylated AKT (p-AKT) in the transfected cells with overexpressed GATA6-AS1 and inhibited PTEN or added with SF1670 (left panels). Quantification of western blot results was shown in the bar graphs (right panels). (d) Cell viability of GC cells in different groups was assessed by CCK-8. (e) Cell proliferation in different groups was evaluated by colony formation assays. (f) Wound healing assays were conducted to assess the migratory capacity of GC cells in different groups. (g) The evaluation of cell migration in different groups through transwell assays was displayed. One-way ANOVA followed by Dunnett’s test. .

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