lncRNA DLX6-AS1 Promotes Myocardial Ischemia-Reperfusion Injury through Mediating the miR-204-5p/FBXW7 Axis

Figure 4

DLX6-AS1 interacted with miR-204-5p and repressed miR-204-5p expression. (a) The predicted binding sites between DLX6-AS1 and miR-204-5p were presented. (b) The interaction between DLX6-AS1 and miR-204-5p in H9c2 cells was verified by luciferase reporter assay. (c) IR rats were established by LAD. Sham-operated rats were served as control. H9c2 cells were subjected to HR. Normal H9c2 cells were served as control. The expression of miR-204-5p and DLX6-AS1 in the myocardial tissues and H9c2 cells was examined by qRT-PCR. (d) RIP assay was carried out utilizing an anti-Ago2 antibody in H9c2 cells transfected with pc-DLX6-AS1 to detect the expression of DLX6-AS1 and miR-204-5p through qRT-PCR. (e) The qRT-PCR was carried out to assess the expression of miR-204-5p in H9c2 cells transfected with si-NC, si-DLX6-AS1, pc-DNA, or pc-DLX6-AS1. vs. NC-mimic, Sham, Control, IgG, si-NC; ^## vs. pc-DNA.

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