Inhibition of Schwann Cell Pyroptosis Promotes Nerve Regeneration in Peripheral Nerve Injury in Rats

Figure 1

Injury induced Schwann cell pyroptosis in peripheral nerves in vivo. (a) The levels of pyroptosis-associated proteins were detected by western blot analysis in the injured sciatic nerve after transection. β-Actin was used as an internal control. (b, c) Levels of N-GSDMD and Cleaved-casp-1 were quantified by densitometric measurement. compared with the control group. Data are expressed as the . All experiments were repeated three times. (d) Representative images of immunofluorescence staining of GSDMD (red) and S100 (green) with DAPI (blue) in sciatic nerve sections after transection. Cells with GSDMD, S100, and DAPI expression (yellow) are labeled with arrowheads. Scale bar, 25 μm. (e, f) Transmission electron microscope observation of the injured sciatic nerve on the 3rd postoperative day. Red arrowhead indicates Schwann cell nucleus pyknosis, and white arrowheads indicate large bubbles emerging from the cytoplasm of Schwann cells. Scale bars: (e) 10 μm and (f) 5 μm.

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