Inhibition of Schwann Cell Pyroptosis Promotes Nerve Regeneration in Peripheral Nerve Injury in Rats

Figure 4

Schwann cell pyroptosis suppressed the function of cocultured DRGns. (a) Schematic for the coculture procedure. (b, c) A representative ordinary optics microscope image of primary DRGns before coculture with Schwann cells. Scale bar, (b) 200 μm and (c) 100 μm. Real-time PCR was performed to detect the mRNA expression of (d) IL-6 and (e) TNF-α. compared with the control group. ^# compared with the LPS+ATP+PBS group. (f) Immunofluorescence analysis of the distribution of NeuN and βIII-Tubulin in DRGns after 24 h of coculture with different groups of Schwann cells, and the nuclei were counterstained using DAPI. Scale bar, 200 μm. (g, h) The average axonal length and body size of DRGns cocultured with different groups of Schwann cells were analyzed. compared with the control group. ^# compared with the LPS+ATP+PBS group. Data are expressed as the .