Research Article

Inhibition of Schwann Cell Pyroptosis Promotes Nerve Regeneration in Peripheral Nerve Injury in Rats

Figure 5

Ac-YVAD-cmk promotes nerve regeneration in peripheral nerve injury. (a) NLRP3, N-GSDMD, and Cleaved-casp-1 levels were detected by western blot analysis. β-Actin was used as an internal control. (b–d) Levels of N-GSDMD, NLRP3, and Cleaved-casp-1 were quantified by densitometric measurement. compared with the control group. # compared with the LPS+ATP+AC group. Data are expressed as the . (e) Immunofluorescence analysis of the distribution of NLRP3 and S100 in sciatic nerve sections. Scale bar, 50 μm. (f) Representative transmission electron microscopic images were selected from three different groups. Scale bars, 10 μm and 2 μm. (g, h) Statistical results of the average myelinated axon diameter and thickness of the myelin sheath in rats from different groups. compared with the control group. # compared with the LPS+ATP+AC group. Data are expressed as the . (i) The sciatic function index (SFI) values in all groups were measured at the predetermined time postoperatively.
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