Penfluroidol Attenuates the Imbalance of the Inflammatory Response by Repressing the Activation of the NLRP3 Inflammasome and Reduces Oxidative Stress via the Nrf2/HO-1 Signaling Pathway in LPS-Induced Macrophages

Figure 2

PF alleviated LPS-induced inflammation in LPS-stimulated macrophages. RAW264.7 and THP-1 cells were treated with PF for 24 hr and stimulated with LPS (1 μg/ml) for 24 hr. (a and b) The level of NO was measured by Griess Reagent. (c–f) The concentrations of IL-6 and TNF-α were detected by ELISA and qRT-PCR in treated RAW264.7 cells. (g–j) The levels of IL-6 and TNF-α were assessed by qRT-PCR and ELISA in treated THP-1 cells. The data are shown as the mean ± SD, and one-way ANOVA was used to compare the differences. , , and .

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