Penfluroidol Attenuates the Imbalance of the Inflammatory Response by Repressing the Activation of the NLRP3 Inflammasome and Reduces Oxidative Stress via the Nrf2/HO-1 Signaling Pathway in LPS-Induced Macrophages

Figure 4

PF reduced oxidative stress in LPS-stimulated macrophages. RAW264.7 and THP-1 cells were treated with PF for 24 hr and stimulated with LPS (1 μg/ml) for 24 hr. (a) The level of ROS was measured by the 2, 7-dichloro-fluorescein diacetate (DCFH-DA) in treated RAW264.7 cells. (b and c) SOD and MDA contents in RAW264.7 cells were detected by MDA and SOD Assay Kits. (d) The level of ROS was measured by the 2, 7-dichloro-fluorescein diacetate (DCFH-DA) in treated THP-1 cells. (e and f) SOD and MDA contents in THP-1 cells were detected by MDA and SOD Assay Kits. The data are shown as the mean ± SD, and one-way ANOVA was used to compare the differences. , and .

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