Sorbitol Destroyed Intestinal Microfold Cells (M Cells) Development through Inhibition of PDE4-Mediated RANKL Expression

Figure 2

Sorbitol regulated M-cell differentiation through PDE4/PKA/CREB signaling-mediated RANKL: (a) after establishing M-cell model, the total protein was extracted from M-cell model treated with 100 mM mannitol or 100 mM sorbitol for 24 hr, western blotting was used to detect RANKL; (b) the M cells was established and treated described in (a), and the concentration of RANKL level in supernatant was measured in indicated group through ELISA assay. Data presented as the mean ± s.e.m. of three independent experiments and were analyzed by t test, ; (c) intestinal organoids culture was performed to visualize the effect of PDE4 inhibition by DIP on sorbitol-mediated M-cell development. Bar: 50 μm. Data presented as the mean ± s.e.m. and were analyzed by one ANOVA, , .

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