A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech

Figure 4

Proliferated cell clusters generated after microinjecting Tpr antisense oligonucleotides into developing Theromyzon tessulatum embryos. (a) Normal, mid-stage 8 embryo fixed $~$48 hours after coinjecting sense oligonucleotide TprC and RDA (red) into the D macromere. (b) Representative embryo fixed $~$48 hours after co-injecting antisense oligonucleotide TprB and FDA (green) into the D macromere. (c) Sampling of embryos from the same experimental group as in (b). Scale bar $=$ 100 $\mu$m (a), (b); 250 $\mu$m (c).

(a)

(b)

(c)