A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech

Figure 6

Time course of cell divisions leading to proliferated cell clusters following Tpr antisense oligonucleotide microinjections into the D macromere. (a) Normal embryo $~$6 hours after microinjection with sense oligonucleotide TprC (co-injected with RDA; red). (b)–(d) Representative atypical cleavages $~$6 hours after microinjection with antisense oligonucleotide TprB (co-injected with FDA; green). (e) Normal embryo $~$12 hours post-injection. (f)–(h) Antisense injected embryos $~$12 hours post-injection. (i) Normal embryo $~$24 hours post-injection. (j)–(k) Antisense injected embryos $~$24 hours post-injection. $\text{Scale}\mathrm{\hspace{0.17em}\hspace{0.17em}}\text{bar}=200$ $\mu$m.

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