A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech
Figure 7
Tpr antisense oligonucleotides affected proteloblast, but not teloblast, cell development. (a) Proteloblast NOPQ (right) was microinjected with Tpr sense oligonucleotide TprC (co-injected with RDA; red) and displayed a normal bandlet pattern, while NOPQ (left) microinjected with Tpr antisense oligonucleotide TprB (co-injected with FDA; green) proliferated abnormally; representative embryo harvested at 36 hours post-injection. (b) M (right) teloblast microinjected with TprC (sense; red) appeared normal 50 hours later; M (left) microinjected with TprB (antisense; green) also appeared normal (the disparity in the extent of each germinal band resulted from the 2 hours delay between sense and antisense microinjections). (f) N (right) teloblast microinjected with TprC (sense; red) appeared normal 72 hours later; N (left) microinjected with TprB (antisense; green) appeared relatively normal. Scale bar 200 m.
