Human Fetal Liver: An In Vitro Model of Erythropoiesis
Figure 4
Analysis of the hemoglobin produced by FL-derived erythroid cells. (a) Quantitative real-time PCR analysis of the globin chains at different times of culture (days 8, 11, 15, and 18). Relative gene expression is given by the method where represents the difference of an unknown sample (days 8, 11, 15, and 18 of culture) versus a control (day 0 of culture) for a target gene (globin genes) normalized to a control gene (18S). (b) HPLC analysis of the hemoglobin (Hb) produced on days 8 (), 16 (), and 19–24 () of erythroid differentiation. Graph and profile show mainly fetal hemoglobin (HbF) including an acetylated fraction.