Research Article

Human Fetal Liver: An In Vitro Model of Erythropoiesis

Figure 4

Analysis of the hemoglobin produced by FL-derived erythroid cells. (a) Quantitative real-time PCR analysis of the globin chains at different times of culture (days 8, 11, 15, and 18). Relative gene expression is given by the 2 Δ Δ C t method where Δ Δ C t represents the difference of an unknown sample (days 8, 11, 15, and 18 of culture) versus a control (day 0 of culture) for a target gene (globin genes) normalized to a control gene (18S). (b) HPLC analysis of the hemoglobin (Hb) produced on days 8 ( 𝑛 = 6 ), 16 ( 𝑛 = 2 ), and 19–24 ( 𝑛 = 5 ) of erythroid differentiation. Graph and profile show mainly fetal hemoglobin (HbF) including an acetylated fraction.
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405429.fig.004b
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