Recovery and Biodistribution of Ex Vivo Expanded Human Erythroblasts Injected into NOD/SCID/IL2R𝜸^null mice

Table 1

Frequency of EBs divided according to their maturation profile at day 10 of HEMA culture seeded with AB and CB MNC and after 4 days of additional culture in EPO alone to induce maturation. The gates used to define the 4 maturation classes used in this study are described in Supplemental Figure 3.

Maturation classes	AB MNC		CB MNC
	Day 10 (%)*	Day 4 + EPO (%)	Day 10 (%)	Day 4 + EPO (%)
Class I (CD36^highCD235a^neg)	17.0±6.2	1.6±0.6	53.8±8.9	11.9±4.8
Class II (CD36^highCD235a^low)	21.7±4.1	9.7±1.1	21.5±6.5	10.6±4.5
Class III (CD36^highCD235a^high)	49.2±2.4	55.1±7.4	9.1±4.6	49.5±6.4
Class IV (CD36^lowCD235a^high)	1.2±1.1	15.8±4.4	0.2±0.6	1.7±1.1

*Percentages were calculated with respect to the total cell population present in culture (EBs and non EBs).