Spleen as a Site for Hematopoiesis of a Distinct Antigen Presenting Cell Type

Figure 2

Classification of DC and myeloid subsets in spleen. Subsets in spleen can be identified flow cytometrically in terms of their phenotype following staining with specific antibody to cell surface markers. The different subsets can be identified based on variant expression of CD11c, CD11b, CD8α and MHC-II. The phenotype of L-DC is shown by comparison with pDC, cDC and a broad monocyte population. The different subsets can also be located in distinct positions on a bivariate CD11c versus CD11b scatter plot.