Feline Neural Progenitor Cells I: Long-Term Expansion under Defined Culture Conditions

Table 1

Cat-specific primers for real-time PCR.

Genes	Description	Forward (5′–3′)	Reverse (5′–3′)	Annealing temperature (°C)	Product size (base pairs)
Nestin	Intermediate filament, Progenitor	CTGGAGCAGGAGAAGGAGAG	GAAGCTGAGGGAAGCCTTG	60	180
Sox-2	Transcription factor, progenitor	ACCAGCTCGCAGACCTACAT	TGGAGTGGGAGGAAGAGGTA	60	154
Vimentin	Intermediate filament, progenitor	ATCCAGGAGCTACAGGCTCA	GGACCTGTCTCCGGTACTCA	60	247
Pax 6	Transcription factor, progenitor	AGGAGGGGGAGAGAATACCA	CTTTCTCGGGCAAACACATC	60	183
Notch1	Surface receptor, progenitor	CAGTGTCTGCAGGGCTACAC	CTCGCACAGAAACTCGTTGA	60	231
CD133	Progenitor	AGGAAGTGCTTTGCGGTCT	TGCCAGTTTCCGAGTCTTTT	60	120
Cyclin D2	Cell cycle protein	CAAGATCACCAACACGGATG	ATATCCCGCACGTCTGTAGG	60	162
Ki-67	Cell cycle protein, proliferation	TCGTCTGAAGCCGGAGTTAT	TCTTCTTTTCCCGATGGTTG	60	150
CD81	Tetraspaniin	CCACAGACCACCAACCTTCT	CAGGCACTGGGACTCCTG	60	156
EGFR	EGF receptor, surface marker	AACTGTGAGGTGGTCCTTGG	CGCAGTCCGGTTTTATTTGT	60	231
FABP7	fatty acid binding protein	TGGAGGCTTTCTGTGCTACC	TGCTTTGTGTCCTGATCACC	60	165
β3-tubulin	Microtubule protein, neural precursor	CATTCTCGTGGACCTTGAGC	GCAGTCGCAATTCTCACATT	60	199
Map2	Microtubule-associated, neuron	ACCTAAGCCATGTGACATCCA	CTCCAGGTACATGGTGAGCA	60	152
GFAP	Intermediate filament, glia	CGGTTTTTGAGGAAGATCCA	TTGGACCGATACCACTCCTC	60	188
AQP4	water channel protein	TACACTGGTGCCAGCATGA	CACCAGCGAGGACAGCTC	60	118
SDF 1	stromal-cell-derived factor-1	ACAGATGTCCTTGCCGATTC	CCACTTCAATTTCGGGTCAA	60	152
CXCR4	fusin	TCTGTGGCAGACCTCCTCTT	TTTCAGCCAACAGCTTCCTT	60	220
Dcx	Doublecortin, neuroblast marker	GGCTGACCTGACTCGATCTC	GCTTTCATATTGGCGGATGT	60	222
Lhx2	Homeobox transcription factor	GATCTGGCGGCCTACAAC	AGGACCCGTTTGGTGAGG	60	224
NCAM (CD56)	Adhesion molecule, surface marker	AGAACAAGGCTGGAGAGCAG	TTTCGGGTAGAAGTCCTCCA	60	172
NogoA	Reticulon 4, surface protein	TTTGCAGTGTTGATGTGGGTA	TAACAGGAACGCTGAAGAGTGA	60	100
nucleostemin	Nucleolar protein	CAGTGGTGTTCAGAGCCTCA	CCGAATGGCTTTGCTGTAA	60	165
Pbx 1	Transcription factor	CTCCGATTACAGAGCCAAGC	GCTGACCATACGCTCGATCT	60	166
β-actin	Housekeeping gene	GCCGTCTTCCCTTCCATC	CTTCTCCATGTCGTCCCAGT	60	168