Minced Umbilical Cord Fragments as a Source of Cells for Orthopaedic Tissue Engineering: An In Vitro Study
Figure 4
Osteogenic differentiation. Alizarin red staining revealing formation of calcium deposition in UC-MSCs (a) and in human BM-MSCs (b); (c) alcaline phosphatase cytoenzymatic staining of UC-MSCs; immunofluorescence for osteocalcin in UC-MSCs cultured in osteogenic medium; (d) osteocalcin stained with primary antibody against and with a secondary antibody anti-mouse (conjugated with Alexa Fluor 488) and merged with DAPI (blue, nuclei), (e) negative control with DAPI and secondary antibody only.
