Effects of Severe Hypoxia on Bone Marrow Mesenchymal Stem Cells Differentiation Potential

Table 1

Sequences of qPCR primers used for the amplification of human mRNA corresponding to adipogenic, osteogenic, and chondrogenic-specific genes.

Gene name	Forward primer (5′-3′)	Reverse primer (5′-3′)	mRNA ID number
Sox9	gtacccgcacttgcacaac	tcgctctcgttcagaagtctc	NM_000346
Col2A1	gtgtcagggccaggatgt	tcccagtgtcacagacacagat	NM_001844
Aggrecan (Agg)	gcctacgaagcaggctatga	gcacgccataggtcctga	BC036445
Col1	gtgatgctggtcctgttggt	caccatcgtgagccttctct	NM_000088
FABP4	ggatgataaactggtggtgga	cacagaatgttgtagagttcaatgc	NM_001442
APM1	ggtgagaaaggagatccaggt	tgctgagcggtatacataggc	NM_004797
LPL	agaacatcccattcactctgc	ccatttgagcttcaacatgagt	NM_000237
ALP	gacggacccgtcactctc	gtgcccgtggtcaattct	NM_000478
OP	cgcagacctgacatccagt	ggctgtcccaatcagaagg	NM_000582
TBP	gcccatagtgatctttgcagt	cgctggaactcgtctcacta	NM_003194