Differentiation of Dental Pulp Stem Cells into Neuron-Like Cells in Serum-Free Medium

Table 1

Primers involved in RT-PCR experiments.

Gene/accession no.	Primers	Sequences	Expected product size (bp)	Annealing temperature (°C)
Cd146 (NM_023061)	Forward	5′-GGACCTTGAGTTTGAGTGG-3′	479	60
	Reverse	5′-CAGTGGTTTGGCTGGAGT-3′
Cd166 (NM_009655)	Forward	5′-AACATGGCGGCTTCAACG-3′	630	61
	Reverse	5′-GACGACACCAGCAACGAG-3′
Nestin (NM_016701)	Forward	5′-CGCTCGGGAGAGTCGCTT-3′	215	64
	Reverse	5′-CCAGTTGCTGCCCACCTTC-3′
Gapdh (NM_008084)	Forward	5′-CAACGGCACAGTCAAGG-3′	717	62
	Reverse	5′-AAGGTGGAAGAGTGGGAG-3′
Tub3 (NM_023279)	Forward	5′-ACGCATCTCGGAGCAGTT-3′	125	61
	Reverse	5′-CGGACACCAGGTCATTCA-3′
Cd31 (NM_001032378.1)	Forward	5′-GGTCTTGTCGCAGTATCAG-3′	355	58
	Reverse	5′-ATGGCAATTATCCGCTCT-3′