Nonviral Gene Delivery of Growth and Differentiation Factor 5 to Human Mesenchymal Stem Cells Injected into a 3D Bovine Intervertebral Disc Organ Culture System
Figure 3
Relative gene expression to untransfected cells after 7, 14, and 21 days (three time points) in monolayer culture (a) and (b) in 3D cell culture using 1.2% alginate beads; gray bars correspond to state of day 0 cells (day 7 in monolayer culture). Bars represent min. to max. with a line at the mean. N = 4 ± SEM. *** denotes significantly different from 1 (untransfected cells) with .
