Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC

Table 1

Composition of growth media for culture of oMSC.

100 mL medium	F2	F10	F2-EGF	F10-EGF	F2-S	F10-S	F2-S-EGF	F10-S-EGF
DMEM 1 g/L glucose [mL]^1	97	89	97	89	56.4	51.6	56.4	51.6
L-Glutamine penicillin streptomycin (LGPS) [mL]^1	1	1	1	1	1	1	1	1
F
FCS [mL]^2	2	10	2	10	2	10	2	10
S
MCDB-201 [mL]^3	—	—	—	—	37.6	34.4	37.6	34.4
ITS + Premix 1x [mL]^3	—	—	—	—	1	1	1	1
Dexamethasone^3, stock 100 mM [µL], final conc. 1 nM	—	—	—	—	1	1	1	1
L-Ascorbic acid^3, stock 500 mM [mL], final conc. 100 µM	—	—	—	—	2	2	2	2
EGF
EGF [ng/mL]^3	—	—	10	10	—	—	10	10

1PAA, Coelbe, Germany.
²Pan Biotech, Aidenbach, Germany.
³Sigma-Aldrich, Steinheim, Germany.