Developing a New Two-Step Protocol to Generate Functional Hepatocytes from Wharton’s Jelly-Derived Mesenchymal Stem Cells under Hypoxic Condition

Table 1

List of primer sequences used in this study.

Gene	Primer sequence (5′ to 3′)	Annealing Temp.	Size; (bp)	Reference
AFP	GCTTGGTGGTGGATGAAACA; TCCTCTGTTATTTGTGGCTTTTG	62	157	[22]
ALB	TGAGAAAACGCCAGTAAGTGAC; TGCGAAATCATCCATAACAGC	62	265	[22]
CYP3A4	CCTTACAT TACACACCCTTTGGAAGT; AGCTCAATGCATGTACAGAATCCCCGGTTA	62	382	[22]
HNF4α	GCCTACCTCAAAGCCATCAT; GACCCTCCCAGCAGCATCTC	62	275	[22]
AAT	ACATTTACCCAAACTGTCCATT; GCTTCAGTCCCTTTCTCGTC	56	183	[23]
TTR	GGTGAATCCAAGTGTCCTCTGAT; GTGACGACAGCCGTGGTGGAA	61	352	[24]
G6P	GCTGGAGTCCTGTCAGGCATTGC; TAGAGCTGAGGCGGAATGGGAG	56	350	[23]
TAT	CCCCTGTGGGTCAGTGTT; GTGCGACATAGGATGCTTTT	56	345	[23]
GAPDH	AGCCACATCGCTCAGACACC; GTACTCAGCGGCCAGCATCG	60	302	[25]

ALB: albumin; AFP: alpha-fetoprotein; AAT: alpha-1-antitrypsin; CYP3A4: cytochrome P450 type 3A4; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; G6P: glucose-6-phosphatase; HNF4α: hepatocyte nuclear factor 4α; TAT: tyrosine aminotransferase; TTR: transthyretin; Temp.: temperature; bp: base pair.