Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105⁺ hMSCs

Figure 1

Characterization of CD105⁺ hMSCs. ((a), (b)) Functional differentiation capacity of freshly isolated CD105⁺ cells was shown by immunostaining of FABP-4 (green) for adipocytes (a) and osteocalcin (green) for osteocytes (b) after 20 days in appropriate differentiation medium. Nuclei were counter stained with DAPI (blue). Scale bars = 50 μm. ((c), (d)) Immunophenotyping of freshly isolated ((c), (d)) and cultured CD105⁺ hMSCs (d) was evaluated by flow cytometry after staining of specific cell surface markers. Corresponding isotype controls were used as negative controls (c). The relative expression values of CD marker expression are shown as mean ± SD; 𝑛=2 (d).

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