Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs
Figure 2
Characterization and transfection optimization of miR/PEI complexes. (a) Condensation of miR/PEI complexes composed of 20 pmol miR and different N/P ratios (ranging between 0.1 and 33) was analysed by gel electrophoresis. miR alone served as positive control. ((b), (c)) Gating strategy of FACS measurements. (b) Untransfected living CD105+ cells were used as negative control. (c) Living CD105+ cells transfected with Cy3-labeled complexes. ((d), (e)) Optimization of miR amounts and N/P ratios for transfection of freshly isolated hMSCs. hMSCs were transfected with Cy3-labeled miR/PEI complexes consisting of three different N/P ratios (N/P 2.5, N/P 10, and N/P 33) and two different miR amounts (5 and 10 pmol). 24 hours after transfection, uptake efficiency (d) and cytotoxicity (e) of complexes were analyzed by flow cytometry. Untransfected cells were used as control. Values are presented as mean ± SEM; ; .
