Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs
Figure 3
Transfection optimization of miR/PEI/MNP complexes. ((a)–(d)) Optimization of MNP amounts for transfection of freshly isolated hMSCs with (+M) and without (−M) the application of a magnetic field. hMSCs were transfected with Cy3-labeled miR/PEI and miR/PEI/MNP complexes composed of an N/P ratio of 10 combined with 5 pmol miR ((a), (b)) or an N/P ratio of 2.5 combined with 10 pmol miR ((c), (d)) and three different MNP amounts (0, 1, or 2 μg/mL MNPs). 24 hours after transfection, uptake efficiency ((a), (c)) and cytotoxicity ((b), (d)) of complexes were analyzed by flow cytometry. Untransfected cells were used as control. Values are presented as mean ± SEM; .
