Effects of Human Mesenchymal Stem Cells Isolated from Wharton’s Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy Model

Figure 2

Biopsy punch for myectomy lesion creating a 5 mm Ø defect in the rat’s tibialis anterior (TA) muscle (a–c). In the MSCFibrin group, the defect was filled with a cellular suspension containing 1 × 10⁶ HMSCs and 30 μL of fibrin glue (Tisseellyo) (d). Preparation of Floseal: the gelatin matrix granules syringe is connected to the syringe containing the thrombin solution and the mixture is transferred back and forth between the syringes for a total of 10 passes (e). Application of Floseal in a 5 mm Ø myectomy defect (f). Preparation of the spherical hydrogel: the polymer solution containing alginate and sodium hyaluronate in a ratio of 1 : 1 (V/V) was inserted into an insulin syringe and a droplet was released into an excess of cerium nitrate solution 135 mM in order to obtain a cross-linked polymer sphere of approximately 60 μL in volume (g–i). Application of the previously prepared spherical hydrogel in a 5 mm Ø myectomy defect (j).

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