Effects of Human Mesenchymal Stem Cells Isolated from Wharton’s Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy Model

Figure 3

ISO 10993-6 scoring for the groups tested at d15. The Control group obtained a score of 14.72. The tested implants can be considered as slight, moderate, severe, and nonirritant depending on the score obtained in comparison to the Control group (a). Collagen fractions for the groups tested at d15 (b). Histological appearance (H&E staining) of a tibialis anterior (TA) muscle with exuberant calcification (arrows) after Floseal implantation (magnification: 40x). Scale bar: 50 μm (c). Scoring system used for biological evaluation of degradable materials taking into account inflammation, neovascularization, fibrosis, and fatty infiltrate-ISO 10993-6 Annex E (d). International Standard classification of the biomaterials attributed by the results obtained through the previous scoring system: the average subtotal inflammation score is multiplied by 2 and added to the average neovascularization, fibrosis, and fatty infiltrate subtotal score. The final score for the tested biomaterial is the value obtained above (or below) the Control score (e). Histological appearance (H&E staining) from Control, Fibrin, MSCFibrin, and ConditionedFibrin TA muscles at d15 and d35 for the assessment of inflammatory status (f).