The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells
Figure 1
Metabolic viability (MTS test) of SH-SY5Y cells seven days after incubation with BMSCs and HUCPVCs CM. Results revealed that, for all CM tested conditions, the secretome of both MSCs populations is able to support neuronal-like cell viability without the use of any other exogenous growth factors. The differences observed towards RA-differentiated cells () are considered to be natural as these cultures were supplemented with 1% of FBS. Concerning statistical differences among time points, BMSCs CM 24 h promoted a significant increase in SH-SY5Y cells viability when compared with the HUCPVCs CM 24 h group () (values are shown as mean ± SEM, ). Symbols correspondence to statistical signification: (1) refers to comparisons between RA-differentiated cells and MSCs CM; (2) # regards the correlation between MSCs CM from the same time point , ).
