Phenotypic and Proteomic Characteristics of Human Dental Pulp Derived Mesenchymal Stem Cells from a Natal, an Exfoliated Deciduous, and an Impacted Third Molar Tooth

Figure 7

WB validation of the selected proteins identified by MALDI-TOF/TOF analysis. Western blot analysis was used for validation of proteomic results. Conventional 12% SDS gels were run with whole cell extracts. (a) Three lanes for SHED, DPSCs, and NDP-MSC are shown for each antibody. Galectin-1, DJ-1, UCHL-1, and HNRNPH1 antibodies were used for validation of expressions of the identified proteins. GAPDH was used for the normalization of each protein sample. (b) Relative protein intensities of bands were measured by Quantity One 1D analysis software (Bio-Rad). Each WB was repeated for three times. (c) Representative images of protein spots selected for WB analysis (protein identities corresponding to SSP numbers can be found in Table 2). (d) Comparative intensity graphs of selected protein spots generated by PDQuest Advance software (Bio-Rad).