Phenotypic and Proteomic Characteristics of Human Dental Pulp Derived Mesenchymal Stem Cells from a Natal, an Exfoliated Deciduous, and an Impacted Third Molar Tooth

Table 2

Protein spots that were identified by MALDI-TOF/TOF (MS/MS) analysis.

SSP	Swiss-Prot accession	Best protein description	Sequence coverage/matches	MASCOT probability/expect (p)	Theoretical; MW (Da)	Experimental p
3001	P 09382	Galectin-1	40%/9 fragment	236/5.10e − 20	14706	5.34
3005	P 06703	Protein S100-A6	24%/7 fragment	124/8.10e − 09	10173	5.33
4003	P 62988	Ubiquitin	31%/5 fragment	151/1.60e − 11	8560	6.56
5002	O 75368	SH3 domain-binding glutamic acid-rich-like protein	22%/5 fragment	181/1.60e − 14	12766	5.22
5001	Q 14019	Coactosin-like protein	28%/7 fragment	93/1.00e − 05	15935	5.54
4001	O 75347	Tubulin-specific chaperone A	48%/10 fragment	295/6.40e − 26	12847	5.25
3003	Q 9NQ39	Putative 40S ribosomal protein S10-like	20%/7 fragment	22/1.10e + 02	20108	10.13
3002	P 63241	Eukaryotic translation initiation factor 5A-1	15%/5 fragment	177/4.00e − 14	16821	5.08
3004	O 95881	Thioredoxin domain-containing protein 12	12%/4 fragment	47/0.41	19194	5.24
3101	P 52815	39S ribosomal protein L12, mitochondrial	15%/4 fragment	164/8.10e − 13	21335	9.04
4101	O 75947	ATP synthase subunit d, mitochondrial	32%/8 fragment	322/1.30e − 28	18480	5.21
4102	P 09936	Ubiquitin carboxyl-terminal hydrolase isozyme L1	22%/8 fragment	212/1.30e − 17	24808	5.33
4002	P 07858	Cathepsin B	5%/1 fragment	107/4.00e − 07	37797	5.88
3201	P 43487	Ran-specific GTPase-activating protein	28%/10 fragment	167/4.00e − 13	23296	5.19
6102	P 32119	Peroxiredoxin-2	34%/15 fragment	536/5.10e − 50	21878	5.66
6104	P 30085	UMP-CMP kinase	13%/5 fragment	140/2.00e − 10	22208	5.44
6202*	P 04792	Heat shock protein beta-1	33%/12 fragment	321/1.60e − 28	22768	5.98
7101	P 00441	Superoxide dismutase Cu-Zn	9%/2 fragment	101/1.60e − 06	15926	5.7
6001	O 15511	Actin related protein 2/3 complex subunit 5	12%/3 fragment	78/0.00035	16310	5.47
7001	Q 12912	Lymphoid-restricted membrane protein	1%/2 fragment	25/67	62069	5.62
7204	P 07339	Cathepsin D	20%/17 fragment	503/1.00e − 46	44524	6.1
7205	Q 99436	Proteasome subunit beta type 7	14%/9 fragment	150/2.00e − 11	29946	7.57
7102	P 09211	Glutathione S-transferase P	16%/5 fragment	215/6.4e − 018	23341	5.43
4201A*	P 07858	Cathepsin B	28%/14 fragment	574/8.10e − 54	37797	5.88
4201B	O 00299	Chloride intracellular channel protein 1	10%/6 fragment	130/2.00e − 09	26906	5.09
8101	P 04792	Heat shock protein beta-1	45%/22 fragment	707/4.00e − 67	22768	5.98
8201	P 30048	Thioredoxin-dependent peroxide reductase, mitochondrial	23%/10 fragment	346/5.10e − 31	27675	7.67
9102	O 95571	Protein ETHE1, mitochondrial	35%/12 fragment	439/2.60e − 40	27855	6.35
9101	Q 99497	Protein DJ-1	33%/10 fragment	239/2.60e − 20	19878	6.33
8202	P 30084	Enoyl-CoA hydratase, mitochondrial	25%/10 fragment	183/1.00e − 14	31367	8.34
9202*	P 60174	Triosephosphate isomerase	64%/24 fragment	872/1.30e − 83	26653	6.45
9203*	P 07339	Cathepsin D	16%/14 fragment	233/1.00e − 19	44524	6.1
8002	P 31949	Protein S100-A11	56%/10 fragment	312/1.30e − 27	11733	6.56
9003	P 49773	Histidine triad nucleotide-binding protein 1	16%/4 fragment	139/2.60e − 10	13793	6.43
9002	Q 969H8	UPF0556 protein C19orf10	30%/12 fragment	437/4.00e − 40	18783	6.2
9201	P 60174	Triosephosphate isomerase	71%/28 fragment	994/8.10e − 96	26653	6.45
9215	P 18669	Phosphoglycerate mutase 1	41%/13 fragment	627/4.00e − 59	28786	6.67
9213	O 00560	Syntenin-1	24%/13 fragment	257/4.00e − 22	32424	7.05
9212	Q 99439	Calponin-2	11%/9 fragment	79/0.00027	33675	6.95
9211	O 14979	Heterogeneous nuclear ribonucleoprotein D-like	15%/13 fragment	361/1.60e − 32	46409	9.59
9209	P 04083	Annexin A1	15%/9 fragment	233/1.00e − 19	38690	6.57
9221	Q 99729	Heterogeneous nuclear ribonucleoprotein A/B	18%/8 fragment	145/6.40e − 11	36202	8.22
9308	Q 14103	Heterogeneous nuclear ribonucleoprotein D0	20%/11 fragment	301/1.60e − 26	38410	7.62
9305	P 60709	Actin, cytoplasmic 1	24%/14 fragment	360/2.00e − 32	41710	5.29
9402	P 06733	Alpha-enolase	24%/19 fragment	494/8.10e − 46	47139	7.01
9401	P 13929	Beta-enolase	14%/8 fragment	165/6.40e − 13	46902	7.59
9401	P 09104	Gamma-enolase	11%/6 fragment	159/2.60e − 12	47239	4.91
9903	Q 8WXF1	Paraspeckle component 1	17%/14 fragment	236/5.10e − 20	58706	6.26
9902	P 04264	Keratin, type II cytoskeletal 1	9%/9 fragment	416/5.10e − 38	65999	8.15
9901	Q 05682	Caldesmon	24%/23 fragment	512/1.30e − 47	93194	5.63
8901	Q 15942	Zyxin	21%/19 fragment	287/4.00e − 25	61238	6.22
8502	P 17661	Desmin	14%/10 fragment	137/4.00e − 10	53503	5.21
7401	P 31943	Heterogeneous nuclear ribonucleoprotein H	49%/29 fragment	966/5.10e − 93	49198	5.89
9603	P 08670	Vimentin	75%/57 fragment	1290/2.00e − 125	53619	5.06
8302	O 14773	Tripeptidyl-peptidase 1	4%/4 fragment	189/2.60e − 15	61210	6.01
8301	P 40121	Macrophage-capping protein	23%/18 fragment	465/6.40e − 43	38494	5.88
5202	P 52907	F-actin-capping protein subunit alpha-1	44%/11 fragment	210/2.00e − 17	32902	5.45
6903	P 11142	Heat shock cognate 71 kDa protein	24%/19 fragment	472/1.3e − 043	70854	5.37
5902	P 10809	60 kDa heat shock protein, mitochondrial	27%/18 fragment	475/6.4e − 044	61016	5.7
5604	Q 16352	Alpha-internexin	5%/3 fragment	72/0.0012	55357	5.34
4902*	Q 96AY3	Peptidyl-prolyl cis-trans isomerase FKBP10	5%/5 fragment	73/0.00093	64204	5.36
4901	P 11021	78 kDa glucose-regulated protein	22%/16 fragment	426/5.1e − 039	72288	5.07
7003	P 15531	Nucleoside diphosphate kinase A	37%/11 fragment	348/3.20e − 31	17138	5.83
9005	Q 01469	Fatty acid-binding protein	27%/5 fragment	246/5.10e − 21	15155	6.6
9204	P 25786	Proteasome subunit alpha type 1	25%/9 fragment	166/5.1e − 013	29537	6.15
9206*	P 60174	Triosephosphate isomerase	30%/8 fragment	116/5.1e − 008	26653	6.45
9010	P 62937	Peptidyl-prolyl cis-trans isomerase A	17%/5 fragment	150/2.00e − 11	18001	7.68

Some of the identified proteins appeared as more than one spot on the gels as indicated by the presence of more than one SSP number.