Long-Term Quantitative Biodistribution and Side Effects of Human Mesenchymal Stem Cells (hMSCs) Engraftment in NOD/SCID Mice following Irradiation
Table 5
Histological analysis of tissue at 120 days after total body irradiation at 3.5 Gray in hMSC injected mice.
Organs
Positive organs
Negative organs
Number of mice
Tumour
Tissue
Number of mice
Tumour
Tissue
Disruption
Abnormality
Disruption
Abnormality
Brain
1
Neg.
Neg.
Neg.
9
Neg.
Neg.
Neg.
Heart
4
Neg.
Neg.
Neg.
6
Neg.
Neg.
Neg.
Lung
2
Neg.
Neg.
Neg.
8
Neg.
Neg.
Neg.
Liver
0
ND
ND
ND
10
Neg.
Neg.
Neg.
Spleen
2
Neg.
Neg.
Neg.
8
Neg.
Neg.
Neg.
Kidney
2
Neg.
Neg.
Neg.
8
Neg.
Neg.
Neg.
Abdominal area
Muscle
1
Neg.
Neg.
Neg.
5
Neg.
Neg.
Neg.
Stomach
0
ND
ND
ND
8
Neg.
Neg.
Neg.
Duodenum
1
Neg.
Neg.
Neg.
9
Neg.
Neg.
Neg.
Jejunum
1
Neg.
Neg.
Neg.
9
Neg.
Neg.
Neg.
Ileum
1
Neg.
Neg.
Neg.
9
Neg.
Neg.
Neg.
Cecum
1
Neg.
Neg.
Neg.
9
Neg.
Neg.
Neg.
Colon proximal
3
Neg.
Neg.
Neg.
7
Neg.
Neg.
Neg.
Colon distal
2
Neg.
Neg.
Neg.
8
Neg.
Neg.
Neg.
Posterior leg (right)
Skin
0
ND
ND
ND
10
Neg.
Neg.
Neg.
Muscle
1
Neg.
Neg.
Neg.
7
Neg.
Neg.
Neg.
Bone
5
Neg.
Neg.
Neg.
5
Neg.
Neg.
Neg.
Bone marrow
4
Neg.
Neg.
Neg.
5
Neg.
Neg.
Neg.
Histological analysis of various radiation-induced tissue damage was performed on all organs collected (heart, lung, liver, kidney, spleen, stomach, intestine in its entirety, muscle and skin of the posterior femur of left leg, and the brain). No tissue damage or structural changes were observed in the studied organs.
