Hematopoietic Origin of Murine Lung Fibroblasts

Figure 1

Adherence culture enriched for the HSC-derived population in the nonadherent fraction of bone marrow. Flow cytometric analysis of CD45 expression in total bone marrow was compared to that in the 96-hour nonadherent bone marrow population. A representative analysis is shown. Panel (a) shows forward and side scatter gate and Panel (b) shows isotype control for CD45 staining gated on the live, propidium iodide (PI) negative population. A representative analysis of CD45 expression by total bone marrow is shown where Panel (c) depicts the forward and side scatter gate and Panel (d) shows CD45 staining gated on the live, PI negative population. Panel (e) depicts the forward scatter and side scatter gate for the nonadherent bone marrow fraction, and Panel (f) shows the CD45 staining gated on the live, PI negative population.

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