Neural Differentiation of Human Adipose Tissue-Derived Stem Cells Involves Activation of the Wnt5a/JNK Signalling

Figure 1

RT-PCR analysis of Wnt pathway-related genes. We demonstrated Wnt genes and Wnt pathway genes by RT-PCR (a–c) and quantified the results (d–f). To figure out the effect of Wnt signal in neural differentiation of stem cell, RT-PCR study was performed using primers of Wnt signal-related genes. (a and d) Most Wnt genes are overexpressed in primary hADSCs whereas the expression of Wnt5a is increased in NI-hADSCs. (b and e) Except Fzd3 and Fzd5, all Wnt receptor and coreceptor genes that are expressed in hADSCs are suppressed in NI-hADSCs. The expression of Fzd3 and Fzd5 is shown in NI-hADSCs. (c and f) The expression of Dvl1 and RYK is decreased in NI-hADSCs. Also, there is no change in the expression of either GSK3β protein or β-catenin. GAPDH was used as a control. The RT-PCR assay was repeated five times independently of different cells and the representative data are shown. The intensity of each gene was normalized to GAPDH. , compared with the primary hADSCs. C, primary hADSCs; NI, NI-hADSCs.

(a)

(b)

(c)

(d)

(e)

(f)