Neural Differentiation of Human Adipose Tissue-Derived Stem Cells Involves Activation of the Wnt5a/JNK Signalling

Figure 2

Real time RT-PCR analysis of Wnt pathway-related genes. Real time RT-PCR was performed to confirm the RT-PCR assay. The expressions of Wnt5a and β-catenin are increased and others are decreased in NI-hADSCs, significantly. Fz3 and Fz5 expressions are not changed. The real time RT-PCR assay was repeated four times independently of different cells and the representative data are shown. The intensity of each gene was normalized to GAPDH. , compared with the primary hADSCs.