Neural Differentiation of Human Adipose Tissue-Derived Stem Cells Involves Activation of the Wnt5a/JNK Signalling

Figure 3

Expression of Wnt signal-related proteins. Western blot analysis demonstrated the Wnt signal in NI-hADSCs (a and b). The expression of Wnt3 is decreased in NI-hADSCs, but interestingly Wnt5a/b expression is upregulated after neural differentiation compared with primary hADSCs. Dvl2 protein is very slightly changed between primary hADSCs and NI-hADSCs. However, the expression of Naked1 is downregulated in NI-hADSCs compared with primary hADSCs. (c–g) Western blot analysis for Wnt signal-related proteins. The expression of phosphor-JNK, which is one of the downstream noncanonical pathways, is upregulated in NI-hADSCs compared with primary hADSCs. Also, ERK1/2 protein level is decreased after neural differentiation. (d–g) Quantified results of protein expression. The western blot was repeated three times independently of different cells and the representative data are shown. The intensity of each gene was normalized to GAPDH or β-catenin. compared with the primary hADSCs.